FCSXpert Solutions: Fluorescence Correlation Spectroscopy Simplified!.
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Mathematics of Autocorrelation

This tutorial will guide you through the detailed derivations of the autocorrelation functions commonly used in Fluorescence correlation spectroscopy (FCS). The first section, Autocorrelation of Fluorescence Intensity, will derive the equation used to calculate an autocorrelation function from fluorescence intensity fluctuations. The remaining sections define fitting models used to obtain physical information from the calculated intensity autocorrelation function.

Calculating the Autocorrelation Function
Fitting Physical Models to the Autocorrelation Function

See our List of FCS Equations for Various Physical Processes for autocorrelation models for the processes above and additional processes.